@article{c1fc313363534e7f9ab9a1352583444e,
title = "Bioluminescent system for dynamic imaging of cell and animal behavior",
abstract = "The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, . ffLuc-cp156, which consists of a yellow variant of . Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic . ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies.",
keywords = "Bioluminescence, Chemiluminescence, Fluorescent protein, Luciferase, Transgenic mouse",
author = "Chikako Hara-Miyauchi and Osahiko Tsuji and Aki Hanyu and Seiji Okada and Akimasa Yasuda and Takashi Fukano and Chihiro Akazawa and Masaya Nakamura and Takeshi Imamura and Yumi Matsuzaki and Okano, {Hirotaka James} and Atsushi Miyawaki and Hideyuki Okano",
note = "Funding Information: We thank K. Hara and H. Mizuno for helpful discussions, S. Nori for technical support, and T. Harada for animal care. Requests for ff Luc vector should be addressed to Atsushi Miyawaki, and requests for ff Luc mice should be addressed to Hideyuki Okano. Atsushi Miyawaki and Hideyuki Okano contributed equally to this paper. This work was supported by Grants from The Japanese Ministry of Education, Culture Sports, Science, and Technology (MEXT) to Atsushi Miyawaki and Hideyuki Okano, and from “ Funding Program for World-leading Innovative R&D on Science and Technology ” to Hideyuki Okano. ",
year = "2012",
month = mar,
day = "9",
doi = "10.1016/j.bbrc.2012.01.141",
language = "English",
volume = "419",
pages = "188--193",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",
}