Bioluminescent system for dynamic imaging of cell and animal behavior

Chikako Hara-Miyauchi, Osahiko Tsuji, Aki Hanyu, Seiji Okada, Akimasa Yasuda, Takashi Fukano, Chihiro Akazawa, Masaya Nakamura, Takeshi Imamura, Yumi Matsuzaki, Hirotaka James Okano, Atsushi Miyawaki, Hideyuki Okano

研究成果: Article査読

43 被引用数 (Scopus)

抄録

The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, . ffLuc-cp156, which consists of a yellow variant of . Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic . ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies.

本文言語English
ページ(範囲)188-193
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
419
2
DOI
出版ステータスPublished - 2012 3月 9

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Bioluminescent system for dynamic imaging of cell and animal behavior」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル