Bipotential murine hemopoietic cell line (NFS-60) that is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin

K. Hara, T. Suda, J. Suda, M. Eguchi, J. N. Ihle, S. Nagata, Y. Miura, M. Saito

研究成果: Article

43 引用 (Scopus)

抄録

NFS-60 cells were previously obtained from leukemia cells that were infected with the Cas-Br-M murine leukemia virus in vivo. We examined the proliferation and differentiation capacity of NFs-60 cells in the presence of native and recombinant (r) interleukin 3 (IL-3), recombinant granulocyte colony-stimulating factor (rG-CSF), recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF), and r-erythropoietin (Ep) using methylcellulose culture methods. This cell line was able to form colonies in response to each hemopoietic factor, but colony formation was rarely seen in their absence. Some populations of NFS-60 cells could differentiate into neutrophils and macrophages in the presence of IL-3 and GM-CSF. Moreover, in the presence of Ep, this cell line formed well-hemoglobinized colonies as well as nonerythroid colonies. In the presence of G-CSF, NFS-60 cells remained in the promyelocytic state. Electron microscopic studies confirmed these morphologies. A single-cell transfer experiment demonstrated that neutrophils, macrophages, and erythroblasts were derived from a single cell. It is concluded that the NFS-60 cell line is a factor-dependent, bipotential hemopoietic cell line.

元の言語English
ページ(範囲)256-261
ページ数6
ジャーナルExperimental Hematology
16
発行部数4
出版物ステータスPublished - 1988

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Interleukin-3
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Erythropoietin
Cell Line
Neutrophils
Macrophages
Murine Leukemia Viruses
Erythroblasts
Methylcellulose
Leukemia
Electrons
Population

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

これを引用

Hara, K., Suda, T., Suda, J., Eguchi, M., Ihle, J. N., Nagata, S., ... Saito, M. (1988). Bipotential murine hemopoietic cell line (NFS-60) that is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin. Experimental Hematology, 16(4), 256-261.

Bipotential murine hemopoietic cell line (NFS-60) that is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin. / Hara, K.; Suda, T.; Suda, J.; Eguchi, M.; Ihle, J. N.; Nagata, S.; Miura, Y.; Saito, M.

:: Experimental Hematology, 巻 16, 番号 4, 1988, p. 256-261.

研究成果: Article

Hara, K, Suda, T, Suda, J, Eguchi, M, Ihle, JN, Nagata, S, Miura, Y & Saito, M 1988, 'Bipotential murine hemopoietic cell line (NFS-60) that is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin', Experimental Hematology, 巻. 16, 番号 4, pp. 256-261.
Hara, K. ; Suda, T. ; Suda, J. ; Eguchi, M. ; Ihle, J. N. ; Nagata, S. ; Miura, Y. ; Saito, M. / Bipotential murine hemopoietic cell line (NFS-60) that is responsive to IL-3, GM-CSF, G-CSF, and erythropoietin. :: Experimental Hematology. 1988 ; 巻 16, 番号 4. pp. 256-261.
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AU - Eguchi, M.

AU - Ihle, J. N.

AU - Nagata, S.

AU - Miura, Y.

AU - Saito, M.

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