Biselyngbyaside, isolated from marine cyanobacteria, inhibits osteoclastogenesis and induces apoptosis in mature osteoclasts

Takayuki Yonezawa, Naomi Mase, Hiroaki Sasaki, Toshiaki Teruya, Shin Ichi Hasegawa, Byung Yoon Cha, Kazumi Yagasaki, Kiyotake Suenaga, Kazuo Nagai, Je Tae Woo

研究成果: Article

34 引用 (Scopus)

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The mass and function of bones depend on the maintenance of a complicated balance between osteoclast-mediated bone resorption and osteoblast-mediated bone formation. An inhibitor of osteoclast differentiation and/or function is expected to be useful for treatment of bone lytic diseases such as osteoporosis, rheumatoid arthritis, and tumor metastasis into bone. Biselyngbyaside is a recently isolated macrolide compound from marine cyanobacteria Lyngbya sp. that shows wide-spectrum cytotoxicity toward human tumor cell lines. In this study, we investigated the effects of biselyngbyaside on osteoclast differentiation and function. Biselyngbyaside inhibited receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis in mouse monocytic RAW264 cells and primary bone marrow-derived macrophages at a low concentration. Similarly, biselyngbyaside suppressed osteoblastic cell-mediated osteoclast differentiation in cocultures. In the RANKL-induced signaling pathway, biselyngbyaside inhibited the expression of c-Fos and NFATc1, which are important transcription factors in osteoclast differentiation. In mature osteoclasts, biselyngbyaside decreased resorption-pit formation. Biselyngbyaside also induced apoptosis accompanied by the induction of caspase-3 activation and nuclear condensation, and these effects were negated by the pancaspase inhibitor z-VAD-FMK. Taken together, the present findings indicate that biselyngbyaside suppresses bone resorption via inhibition of osteoclastogenesis and induction of apoptosis. Thus, biselyngbyaside may be useful for the prevention of bone lytic diseases.

元の言語English
ページ(範囲)440-448
ページ数9
ジャーナルJournal of Cellular Biochemistry
113
発行部数2
DOI
出版物ステータスPublished - 2012 2

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Yonezawa, T., Mase, N., Sasaki, H., Teruya, T., Hasegawa, S. I., Cha, B. Y., Yagasaki, K., Suenaga, K., Nagai, K., & Woo, J. T. (2012). Biselyngbyaside, isolated from marine cyanobacteria, inhibits osteoclastogenesis and induces apoptosis in mature osteoclasts. Journal of Cellular Biochemistry, 113(2), 440-448. https://doi.org/10.1002/jcb.23213