Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus

Masanori Matsuura, Mina Yamada, Yoko Saikawa, Kazuo Miyairi, Toshikatsu Okuno, Katsuhiro Konno, Jun'ichi Uenishi, Kimiko Hashimoto, Masaya Nakata

研究成果: Article査読

8 被引用数 (Scopus)

抄録

A toxic protein, called bolevenine, was isolated from the toxic mushroom Boletus venenatus based on its lethal effects on mice. On SDS-PAGE, in either the presence or absence of 2-mercaptoethanol, this protein showed a single band of ∼12 kDa. In contrast, based on gel filtration and MALDI-TOFMS, its relative molecular mass was estimated to be ∼30 kDa and ∼33 kDa, respectively, indicating that the protein consists of three identical subunits. This toxin exhibited its lethal activity following injection at 10 mg/kg into mice. The N-terminal amino acid sequence was determined up to 18, and found to be similar to the previously reported bolesatine, a toxic compound isolated from Boletus satanas.

本文言語English
ページ(範囲)893-898
ページ数6
ジャーナルPhytochemistry
68
6
DOI
出版ステータスPublished - 2007 3

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 植物科学
  • 園芸学

フィンガープリント

「Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル