Bone marrow long label-retaining cells reside in the sinusoidal hypoxic niche

Yoshiaki Kubota, Keiyo Takubo, Toshio Suda

研究成果: Article査読

116 被引用数 (Scopus)

抄録

In response to changing signals, quiescent hematopoietic stem cells (HSCs) can be induced to an activated cycling state and provide multi-lineage hematopoietic cells to the whole body via blood vessels. However, the precise localization of quiescent HSCs in bone marrow microenvironment is not fully characterized. Here, we performed whole-mount immunostaining of bone marrow and found that BrdU label-retaining cells (LRCs) definitively reside in the sinusoidal hypoxic zone distant from the "vascular niche". Although LRCs expressed very low level of a well-known HSC marker, c-kit in normal circumstances, myeloablation by 5-FU treatment caused LRCs to abundantly express c-kit and proliferate actively. These results demonstrate that bone marrow LRCs reside in the sinusoidal hypoxic niche, and function as a regenerative cell pool of HSCs.

本文言語English
ページ(範囲)335-339
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
366
2
DOI
出版ステータスPublished - 2008 2月 8

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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