Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity

Takuhiro Matsumura, Yo Sugawara, Masahiro Yutani, Sho Amatsu, Hideo Yagita, Tomoko Kohda, Shin Ichi Fukuoka, Yutaka Nakamura, Shinji Fukuda, Koji Hase, Hiroshi Ohno, Yukako Fujinaga

研究成果: Article査読

50 被引用数 (Scopus)

抄録

To cause food-borne botulism, botulinum neurotoxin (BoNT) in the gastrointestinal lumen must traverse the intestinal epithelial barrier. However, the mechanism by which BoNT crosses the intestinal epithelial barrier remains unclear. BoNTs are produced along with one or more non-toxic components, with which they form progenitor toxin complexes (PTCs). Here we show that serotype A1 L-PTC, which has high oral toxicity and makes the predominant contribution to causing illness, breaches the intestinal epithelial barrier from microfold (M) cells via an interaction between haemagglutinin (HA), one of the non-toxic components, and glycoprotein 2 (GP2). HA strongly binds to GP2 expressed on M cells, which do not have thick mucus layers. Susceptibility to orally administered L-PTC is dramatically reduced in M-cell-depleted mice and GP2-deficient (Gp2-/-) mice. Our finding provides the basis for the development of novel antitoxin therapeutics and delivery systems for oral biologics.

本文言語English
論文番号6255
ジャーナルNature communications
6
DOI
出版ステータスPublished - 2015 2 17

ASJC Scopus subject areas

  • 化学 (全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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