Brachyury downstream notochord differentiation in the ascidian embryo

Hiroki Takahashi, Kohji Hotta, Albert Erives, Anna Di Gregorio, Robert W. Zeller, Michael Levine, Nori Satoh

研究成果: Article査読

163 被引用数 (Scopus)

抄録

The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform endoderm into notochord. Subtractive hybridization screens were conducted to identify potential Brachyury target genes that are induced upon Ci-Bra misexpression. Of 501 independent cDNA clones that were surveyed, 38 were specifically expressed in notochord cells. These potential Ci-Bra downstream genes appear to encode a broad spectrum of divergent proteins associated with notochord formation.

本文言語English
ページ(範囲)1519-1523
ページ数5
ジャーナルGenes and Development
13
12
DOI
出版ステータスPublished - 1999 6 15
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 発生生物学

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