抄録
The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform endoderm into notochord. Subtractive hybridization screens were conducted to identify potential Brachyury target genes that are induced upon Ci-Bra misexpression. Of 501 independent cDNA clones that were surveyed, 38 were specifically expressed in notochord cells. These potential Ci-Bra downstream genes appear to encode a broad spectrum of divergent proteins associated with notochord formation.
本文言語 | English |
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ページ(範囲) | 1519-1523 |
ページ数 | 5 |
ジャーナル | Genes and Development |
巻 | 13 |
号 | 12 |
DOI | |
出版ステータス | Published - 1999 6月 15 |
外部発表 | はい |
ASJC Scopus subject areas
- 遺伝学
- 発生生物学