Brain nitrite production during global ischemia and reperfusion: An in vivo microdialysis study

Mamoru Shibata, Nobuo Araki, Junichi Hamada, Takahiro Sasaki, Kunio Shimazu, Yasuo Fukuuchi

研究成果: Article

51 引用 (Scopus)


Nitric oxide (NO) is considered to be associated with the pathogenesis of cerebral ischemic injury. In the present study, NO production was continuously monitored employing in vivo microdialysis. A microdialysis probe was inserted into the striatum. Levels of the major NO metabolite, NO2- in the dialysate were determined using the Griess reaction. Rats were subjected to global cerebral ischemia produced by occlusion of both common carotid arteries together with induced hypotension. Cerebral ischemia induced a decrease in NO production, which was interrupted by a transient increase in NO synthesis. This increment was abolished in the presence of a NO synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), suggesting that NO synthase activity is transiently activated during ischemia. Following reperfusion, NO synthesis was enhanced. To our knowledge, this is the first report concerning the continuous temporal profile of NO production during global cerebral ischemia and reperfusion.

ジャーナルBrain Research
出版物ステータスPublished - 1996 9 23


ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology