Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest-like precursors

Yuzo Takayama, Tamami Wakabayashi, Hiroko Kushige, Yutaka Saito, Yoichiro Shibuya, Shinsuke Shibata, Wado Akamatsu, Hideyuki Okano, Yasuyuki S. Kida

研究成果: Article

5 引用 (Scopus)

抄録

In this study, we propose a novel method for inducing neuronal cells by briefly exposing them to small-molecule cocktails in a step-by-step manner. Global gene expression analysis with immunohistochemical staining and calcium flux assays reveal the generation of neurons from mouse embryonic fibroblasts. In addition, time-lapse imaging of neural precursor-specific enhancer expression and global gene expression analyses show that the neurons are generated by passing through a neural crest-like precursor stage. Consistent with these results, the neural crest-like cells are able to differentiate into neural crest lineage cells, such as sympathetic neurons, adipocytes, osteocytes, and smooth muscle cells. Therefore, these results indicate that brief exposure to chemical compounds could expand and induce a substantial multipotent cell population without viral transduction.

元の言語English
ジャーナルFEBS Letters
DOI
出版物ステータスAccepted/In press - 2017

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Embryonic Induction
Neural Crest
Fibroblasts
Neurons
Gene expression
Molecules
Cells
Time-Lapse Imaging
Gene Expression
Osteocytes
Chemical compounds
Adipocytes
Smooth Muscle Myocytes
Muscle
Assays
Staining and Labeling
Fluxes
Calcium
Imaging techniques
Population

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest-like precursors. / Takayama, Yuzo; Wakabayashi, Tamami; Kushige, Hiroko; Saito, Yutaka; Shibuya, Yoichiro; Shibata, Shinsuke; Akamatsu, Wado; Okano, Hideyuki; Kida, Yasuyuki S.

:: FEBS Letters, 2017.

研究成果: Article

Takayama, Yuzo ; Wakabayashi, Tamami ; Kushige, Hiroko ; Saito, Yutaka ; Shibuya, Yoichiro ; Shibata, Shinsuke ; Akamatsu, Wado ; Okano, Hideyuki ; Kida, Yasuyuki S. / Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest-like precursors. :: FEBS Letters. 2017.
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AU - Takayama, Yuzo

AU - Wakabayashi, Tamami

AU - Kushige, Hiroko

AU - Saito, Yutaka

AU - Shibuya, Yoichiro

AU - Shibata, Shinsuke

AU - Akamatsu, Wado

AU - Okano, Hideyuki

AU - Kida, Yasuyuki S.

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