Bronchiolar epithelial catalase is diminished in smokers with mild COPD

Tomoko Betsuyaku, Satoshi Fuke, Takashi Inomata, Kichizo Kaga, Toshiaki Morikawa, Nao Odajima, Tracy Adair-Kirk, Masaharu Nishimura

研究成果: Article査読

12 被引用数 (Scopus)

抄録

This study aimed to investigate bronchiolar catalase expression and its relationship with smoking and/or chronic obstructive pulmonary disease (COPD) in humans and to determine the dynamic change of bronchiolar catalase expression in response to cigarette smoke in mice. Lung tissue was obtained from 36 subjects undergoing surgery for peripheral tumours, consisting of lifelong nonsmokers and smokers with or without COPD. Male C57BL/6 mice were subjected to cigarette smoke exposure for up to 3 months followed by a 28-day cessation period. We quantified bronchiolar catalase mRNA using laser capture microdissection and quantitative reverse transcription-polymerase chain reaction. C22 club cells (Clara cells) in culture were exposed to cigarette smoke extract and monitored for viability when catalase expression was decreased by siRNA. Catalase was decreased at mRNA and protein levels in bronchiolar epithelium in smokers with COPD. In mice, bronchiolar catalase is temporarily upregulated at 1 day after cigarette smoke exposure but is downregulated by repeated cigarette smoke exposure, and is not restored long after withdrawal once emphysema is developed. Decreasing catalase expression in C22 cells resulted in greater cigarette smoke extract-induced cell death. Bronchiolar catalase reduction is associated with COPD. Regulation of catalase depends on the duration of cigarette smoke exposure, and plays a critical role for protection against cigarette smoke-induced cell damage.

本文言語English
ページ(範囲)42-53
ページ数12
ジャーナルEuropean Respiratory Journal
42
1
DOI
出版ステータスPublished - 2013 7 1

ASJC Scopus subject areas

  • 呼吸器内科

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