Bubble-Assisted in-Situ Re-Formation of Artificial Bilayer

Izumi Hashimoto, Hirotaka Sugiura, Toshihisa Osaki, Tetsuya Yamada, Hisatoshi Mimura, Norihisa Miki, Shoji Takeuchi

研究成果: Conference contribution

抄録

This paper proposes a technique to reconstitute a lipid bilayer membrane by injecting an air bubble onto the raptured spot. Our group has been studying the artificial bilayer that can host membrane proteins and can be a fundamental to analyze their functions. The only drawback of the lipid bilayer formed at the interface of two water-in-oil droplets is its sustainability, namely, the bilayer often collapses and the droplets merges by the disturbance like self-exerted oscillation or sloshing. To overcome this issue, the newly proposed technique utilizes a bubble at approximately 4μL in volume. When the bubble is injected and retracted, the new artificial bilayer is reconstituted. The major advantage of this technique is the in-situ reconstitution of the bilayer during the electrical measurement of the membrane proteins. The proposed technique was successfully verified by confirming the emergence of nanopore membrane proteins.

本文言語English
ホスト出版物のタイトル33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
出版社Institute of Electrical and Electronics Engineers Inc.
ページ1022-1023
ページ数2
ISBN(電子版)9781728135809
DOI
出版ステータスPublished - 2020 1
イベント33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada
継続期間: 2020 1 182020 1 22

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
2020-January
ISSN(印刷版)1084-6999

Conference

Conference33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
国/地域Canada
CityVancouver
Period20/1/1820/1/22

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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