C-mannosylation of R-spondin3 regulates its secretion and activity of Wnt/β-catenin signaling in cells

Miho Fujiwara, Shintaro Kato, Yuki Niwa, Takehiro Suzuki, Miyu Tsuchiya, Yukiko Sasazawa, Naoshi Dohmae, Siro Simizu

研究成果: Article査読

16 被引用数 (Scopus)

抄録

R-spondin3 (Rspo3) is a secreted protein, which acts as an agonist of canonical Wnt/β-catenin signaling that plays an important role in embryonic development and homeostasis. In this study, we focused on C-mannosylation, a unique type of glycosylation, of human Rspo3. Rspo3 has two putative C-mannosylation sites at Trp153 and Trp156; however, it had been unclear whether these sites are C-mannosylated or not. We demonstrated that Rspo3 was C-mannosylated at both Trp153 and Trp156 by mass spectrometry. Using C-mannosylation-defective Rspo3 mutant-overexpressing cell lines, we found that C-mannosylation of Rspo3 promotes its secretion and activates Wnt/β-catenin signaling.

本文言語English
ページ(範囲)2639-2649
ページ数11
ジャーナルFEBS Letters
DOI
出版ステータスPublished - 2016 8 1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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