Calcium regulates caveolin-1 expression at the transcriptional level

Xiao Yan Yang, Cheng Cheng Huang, Qi Ming Kan, Yan Li, Dan Liu, Xue Cheng Zhang, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Caveolin-1, an indispensable component of caveolae serving as a transformation suppressor protein, is highly expressed in poorly metastatic mouse osteosarcoma FBJ-S1 cells while highly metastatic FBJ-LL cells express low levels of caveolin-1. Calcium concentration is higher in FBJ-S1 cells than in FBJ-LL cells; therefore, we investigated the possibility that calcium signaling positively regulates caveolin-1 in mouse FBJ-S1 cells. When cells were treated with the calcium channel blocker nifedipine, cyclosporin A (a calcineurin inhibitor), or INCA-6 (a nuclear factor of activated T-cells [NFAT] inhibitor), caveolin-1 expression at the mRNA and protein levels decreased. RNA silencing of voltage-dependent L-type calcium channel subunit alpha-1C resulted in suppression of caveolin-1 expression. This novel caveolin-1 regulation pathway was also identified in mouse NIH 3T3 cells and Lewis lung carcinoma cells. These results indicate that caveolin-1 is positively regulated at the transcriptional level through a novel calcium signaling pathway mediated by L-type calcium channel/Ca2+/calcineurin/NFAT.

本文言語English
ページ(範囲)334-341
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
426
3
DOI
出版ステータスPublished - 2012 9 28

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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