Calcium‐Independent Release of Acetylcholine from Stable Cell Lines Expressing Mouse Choline Acetyltransferase cDNA

Hidemi Misawa, Ryosuke Takahashi, Takeo Deguchi

研究成果: Article

18 引用 (Scopus)

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Abstract: Stably transfected cells expressing mouse choline acetyltransferase (ChAT) cDNA were established, and the synthesis and release of acetylcholine (ACh) were examined. A cDNA clone coding for mouse ChAT was inserted into an expression vector (pEF321) containing a promoter for human elongation factor 1α to construct pEFmChAT. Neuronal (NG108‐15, NS20Y, N1E115, and Neuro2A) and nonneuronal cell lines (L cells and NIH3T3) were transfected with pEFmChAT, and the cell lines that stably expressed high ChAT activity were selected. These cells expressed the 66‐kDa ChAT protein and accumulated ACh mostly in the cytosol. The concentration of intracellular ACh in the cells increased upon raising the choline level in the medium. The cells continuously released ACh in a Ca2+‐independent fashion. Neither high K+ nor calcium ionophore stimulated release of ACh from the cells.

元の言語English
ページ(範囲)465-470
ページ数6
ジャーナルJournal of Neurochemistry
62
発行部数2
DOI
出版物ステータスPublished - 1994 2
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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