Cardiac neural crest cells contribute to the dormant multipotent stem cell in the mammalian heart

Yuichi Tomita, Keisuke Matsumura, Yoshio Wakamatsu, Yumi Matsuzaki, Isao Shibuya, Haruko Kawaguchi, Masaki Ieda, Sachiko Kanakubo, Takuya Shimazaki, Satoshi Ogawa, Noriko Osumi, Hideyuki Okano, Keiichi Fukuda

研究成果: Article査読

273 被引用数 (Scopus)


Arodent cardiac side population cell fraction formed clonal spheroids in serum-free medium, which expressed nestin, Musashi-1, and multidrug resistance transporter gene 1, markers of undifferentiated neural precursor cells. These markers were lost following differentiation, and were replaced by the expression of neuron-, glial-, smooth muscle cell-, or cardiomyocyte-specific proteins. Cardiosphere-derived cells transplanted into chick embryos migrated to the truncus arteriosus and cardiac outflow tract and contributed to dorsal root ganglia, spinal nerves, and aortic smooth muscle cells. Lineage studies using double transgenic mice encoding protein 0-Cre/Floxed-EGFP revealed undifferentiated and differentiated neural crest-derived cells in the fetal myocardium. Undifferentiated cells expressed GATA-binding protein 4 and nestin, but not actinin, whereas the differentiated cells were identified as cardiomyocytes. These results suggest that cardiac neural crest-derived cells migrate into the heart, remain there as dormant multipotent stem cells-and under the right conditions-differentiate into cardiomyocytes and typical neural crest-derived cells, including neurons, glia, and smooth muscle.

ジャーナルJournal of Cell Biology
出版ステータスPublished - 2005 9月 26

ASJC Scopus subject areas

  • 細胞生物学


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