Cardiomyocytes undergo cells division following myocardial infarction is a spatially and temporally restricted event in rats

Shinsuke Yuasa, Keiichi Fukuda, Yuichi Tomita, Jun Fujita, Masaki Ieda, Satoko Tahara, Yuji Itabashi, Takashi Yagi, Haruko Kawaguchi, Yasuyo Hisaka, Satoshi Ogawa

研究成果: Article

15 引用 (Scopus)

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Dividing cardiomyocytes are observed in autopsied human hearts following recent myocardial infarction, however there is a lack of information in the literature on the division of these cells. In this study we used a rat model to investigate how and when adult mammalian cardiomyocytes proliferate by cell division after myocardial infarction. Myocardial infarction was induced in Wistar rats by ligation of the left coronary artery. The rats were sacrificed periodically up to 28 days following induced myocardial infarction, and the hearts subjected to microscopic investigation. Cardiomyocytes entering the cell cycle were assayed by observation of nuclear morphology and measuring expression of Ki-67, a proliferating cell marker. Ki-67 positive cardiomyocytes and dividing nuclei were observed initially after 1 day. After 2 days dividing cells gradually increased in number at the ischemic border zone, reaching a peak increase of 1.12% after 3 days, then gradually decreasing in number. Dividing nuclei increased at the ischemic border zone after 3 days, peaked by 0.14% at day 5, and then decreased. In contrast, Ki-67 positive cells and dividing nuclei were limited in number in the non-ischemic area throughout all experiments. In conclusion, mitogenic cardiomyocytes are present in the adult rat heart following myocardial infarction, but were spatially and temporally restricted.

元の言語English
ページ(範囲)177-181
ページ数5
ジャーナルMolecular and Cellular Biochemistry
259
発行部数1-2
DOI
出版物ステータスPublished - 2004 4 1

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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