Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional synapse organizer

Keiko Matsuda, Eriko Miura, Taisuke Miyazaki, Wataru Kakegawa, Kyoichi Emi, Sakae Narumi, Yugo Fukazawa, Aya Ito-lshida, Tetsuro Kondo, Ryuichi Shigemoto, Masahiko Watanabe, Michisuke Yuzaki

研究成果: Article査読

207 被引用数 (Scopus)

抄録

Cbln1, secreted from cerebellar granule cells, and the orphan glutamate receptor 52 (GluD2), expressed by Purkinje cells, are essential for synapse integrity between these neurons in adult mice. Nevertheless, no endogenous binding partners for these molecules have been identified. We found that Cblnl binds directly to the N-terminal domain of GluD2. GluD2 expression by postsynaptic cells, combined with exogenously applied Cbln1, was necessary and sufficient to induce new synapses in vitro and in the adult cerebellum in vivo. Further, beads coated with recombinant Cbln1 directly induced presynaptic differentiation and indirectly caused clustering of postsynaptic molecules via GluD2. These results indicate that the Cbln1-GluD2 complex is a unique synapse organizer that acts bidirectionally on both pre- and postsynaptic components.

本文言語English
ページ(範囲)363-368
ページ数6
ジャーナルScience
328
5976
DOI
出版ステータスPublished - 2010 4 16

ASJC Scopus subject areas

  • General

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