CD9 amino acids critical for upregulation of diphtheria toxin binding

Hidetoshi Hasuwa, Yuji Shishido, Ayano Yamazaki, Terukazu Kobayashi, Xiaochun Yu, Eisuke Mekada

研究成果: Article

29 引用 (Scopus)

抄録

CD9 associates with a diphtheria toxin receptor (DTR) that is identical to the membrane-anchored form of heparin-binding EGF-like growth factor. We determined the region of CD9 important for upregulation activity. Human and monkey CD9 upregulates DT binding activity of DTR, while mouse CD9 has no upregulation activity. Transfection of chimeric constructs comprising monkey and mouse CD9s showed that the human sequence between Ala156 and Asp183 is essential for the upregulation activity. Studies of mutants, replacing a single amino acid within the region between Ala156 and Asp183 of monkey CD9 with the corresponding amino acid residue in mouse CD9, revealed that substitution of Gly158 is critical for the reduction of the upregulation activity and secondly for the substitution of Val159 and Thr175. These three amino acid residues were deduced to be located on the head domain of the second extracellular loop, suggesting that interactions of CD9 with DTR or DT at the domain containing these three amino acids were important for the upregulation of DT binding.

元の言語English
ページ(範囲)782-790
ページ数9
ジャーナルBiochemical and Biophysical Research Communications
289
発行部数4
DOI
出版物ステータスPublished - 2001 12 14
外部発表Yes

Fingerprint

Diphtheria Toxin
Up-Regulation
Amino Acids
Haplorhini
Substitution reactions
Thermodynamic properties
Membranes
Transfection
Heparin-binding EGF-like Growth Factor
Head

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

CD9 amino acids critical for upregulation of diphtheria toxin binding. / Hasuwa, Hidetoshi; Shishido, Yuji; Yamazaki, Ayano; Kobayashi, Terukazu; Yu, Xiaochun; Mekada, Eisuke.

:: Biochemical and Biophysical Research Communications, 巻 289, 番号 4, 14.12.2001, p. 782-790.

研究成果: Article

Hasuwa, Hidetoshi ; Shishido, Yuji ; Yamazaki, Ayano ; Kobayashi, Terukazu ; Yu, Xiaochun ; Mekada, Eisuke. / CD9 amino acids critical for upregulation of diphtheria toxin binding. :: Biochemical and Biophysical Research Communications. 2001 ; 巻 289, 番号 4. pp. 782-790.
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AU - Yu, Xiaochun

AU - Mekada, Eisuke

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