cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene

Hidehiro Yokoji, Takeshi Ariyama, Ryosuke Takahashi, Johji Inazawa, Hidemi Misawa, Takeo Deguchi

研究成果: Article

2 引用 (Scopus)

抄録

Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5′-untranslated region, a 600-bp coding region and a 1207-bp 3′-untranslated region containing four ATTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5′ RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.

元の言語English
ページ(範囲)175-178
ページ数4
ジャーナルNeuroscience Letters
185
発行部数3
DOI
出版物ステータスPublished - 1995 2 13
外部発表Yes

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Ciliary Neurotrophic Factor
Organism Cloning
Complementary DNA
Genes
Chromosomes, Human, Pair 11
Polyadenylation
5' Untranslated Regions
3' Untranslated Regions
Sciatic Nerve
Fluorescence In Situ Hybridization
Gene Library
Methionine
Introns
Sequence Analysis
DNA

ASJC Scopus subject areas

  • Neuroscience(all)

これを引用

cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene. / Yokoji, Hidehiro; Ariyama, Takeshi; Takahashi, Ryosuke; Inazawa, Johji; Misawa, Hidemi; Deguchi, Takeo.

:: Neuroscience Letters, 巻 185, 番号 3, 13.02.1995, p. 175-178.

研究成果: Article

Yokoji, Hidehiro ; Ariyama, Takeshi ; Takahashi, Ryosuke ; Inazawa, Johji ; Misawa, Hidemi ; Deguchi, Takeo. / cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene. :: Neuroscience Letters. 1995 ; 巻 185, 番号 3. pp. 175-178.
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AU - Misawa, Hidemi

AU - Deguchi, Takeo

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