cDNA expression cloning of the 85-kDa protein overexpressed in adriamycin-resistant cells

Kohji Noguchi, Mikihiko Naito, Ken Ichi Tezuka, Shigetaka Ishii, Hiroyuki Seimiya, Yoshikazu Sugimoto, Egon Amann, Takashi Tsuruo

研究成果: Article査読

15 被引用数 (Scopus)

抄録

An 85-kDa cell surface protein recognized by monoclonal antibody MRK-20 was identified in adriamycin-resistant tumor cells. The expression of the 85-kDa protein has recently been reported to be associated with the differentiation of hematopoietic cells. However, the primary structure of the 85-kDa protein has not been determined. To clarify its primary structure, we carried out cDNA expression cloning of the 85-kDa protein with monoclonal antibody MRK-20. We found that the 85-kDa protein is identical to CD36 (GP VI), but at least two species of transcript exist in the tumor cells and one of these transcripts has a novel sequence at the 3′-region. The transcript with the novel sequence at the 3′-region was found to be expressed during the differentiation of hematopoietic cells.

本文言語English
ページ(範囲)88-95
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
192
1
DOI
出版ステータスPublished - 1993 4月 15

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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