TY - JOUR
T1 - cDNA expression cloning of the 85-kDa protein overexpressed in adriamycin-resistant cells
AU - Noguchi, Kohji
AU - Naito, Mikihiko
AU - Tezuka, Ken Ichi
AU - Ishii, Shigetaka
AU - Seimiya, Hiroyuki
AU - Sugimoto, Yoshikazu
AU - Amann, Egon
AU - Tsuruo, Takashi
PY - 1993/4/15
Y1 - 1993/4/15
N2 - An 85-kDa cell surface protein recognized by monoclonal antibody MRK-20 was identified in adriamycin-resistant tumor cells. The expression of the 85-kDa protein has recently been reported to be associated with the differentiation of hematopoietic cells. However, the primary structure of the 85-kDa protein has not been determined. To clarify its primary structure, we carried out cDNA expression cloning of the 85-kDa protein with monoclonal antibody MRK-20. We found that the 85-kDa protein is identical to CD36 (GP VI), but at least two species of transcript exist in the tumor cells and one of these transcripts has a novel sequence at the 3′-region. The transcript with the novel sequence at the 3′-region was found to be expressed during the differentiation of hematopoietic cells.
AB - An 85-kDa cell surface protein recognized by monoclonal antibody MRK-20 was identified in adriamycin-resistant tumor cells. The expression of the 85-kDa protein has recently been reported to be associated with the differentiation of hematopoietic cells. However, the primary structure of the 85-kDa protein has not been determined. To clarify its primary structure, we carried out cDNA expression cloning of the 85-kDa protein with monoclonal antibody MRK-20. We found that the 85-kDa protein is identical to CD36 (GP VI), but at least two species of transcript exist in the tumor cells and one of these transcripts has a novel sequence at the 3′-region. The transcript with the novel sequence at the 3′-region was found to be expressed during the differentiation of hematopoietic cells.
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U2 - 10.1006/bbrc.1993.1385
DO - 10.1006/bbrc.1993.1385
M3 - Article
C2 - 7682812
AN - SCOPUS:0027161017
VL - 192
SP - 88
EP - 95
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -