Cell adhesion to ephrinb2 is induced by EphB4 independently of its kinase activity

Hiroshi Sakamoto; Xiu Qin Zhang; Souichi Suenobu; Kazuyuki Ohbo; Minetaro Ogawa; Toshio Suda

doi:10.1016/j.bbrc.2004.07.016

Cell adhesion to ephrinb2 is induced by EphB4 independently of its kinase activity

Hiroshi Sakamoto, Xiu Qin Zhang, Souichi Suenobu, Kazuyuki Ohbo, Minetaro Ogawa, Toshio Suda

坂口光洋記念講座(発生・分化生物学)

研究成果: Article › 査読

7 被引用数 (Scopus)

抄録

Cell to cell interaction in bone marrow is crucial for differentiation of hematopoietic cells. We have shown that EphB4 receptor is expressed in erythroid progenitor and its activation accelerates erythroid differentiation. To elucidate the role of EphB4 activation in erythropoiesis, we analyzed effects of EphB4 on cell adhesive pathways. Cell adhesion with the extension of filopodial pseudopod was observed by EphB4 activation. EphB4 activation also enhanced an effect of fibronectin-mediated adhesive pathway along with formation of the c-Cbl/CrkL complex. The tyrosine kinase activity of EphB4 was dispensable for those phenomena. These results suggest that activation of EphB4 participates in adhesive but not repulsive signals independently of its tyrosine kinase activity in hematopoietic cells.

本文言語	English
ページ（範囲）	681-687
ページ数	7
ジャーナル	Biochemical and Biophysical Research Communications
巻	321
号	3
DOI	https://doi.org/10.1016/j.bbrc.2004.07.016
出版ステータス	Published - 2004 8 27

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

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10.1016/j.bbrc.2004.07.016

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title = "Cell adhesion to ephrinb2 is induced by EphB4 independently of its kinase activity",

abstract = "Cell to cell interaction in bone marrow is crucial for differentiation of hematopoietic cells. We have shown that EphB4 receptor is expressed in erythroid progenitor and its activation accelerates erythroid differentiation. To elucidate the role of EphB4 activation in erythropoiesis, we analyzed effects of EphB4 on cell adhesive pathways. Cell adhesion with the extension of filopodial pseudopod was observed by EphB4 activation. EphB4 activation also enhanced an effect of fibronectin-mediated adhesive pathway along with formation of the c-Cbl/CrkL complex. The tyrosine kinase activity of EphB4 was dispensable for those phenomena. These results suggest that activation of EphB4 participates in adhesive but not repulsive signals independently of its tyrosine kinase activity in hematopoietic cells.",

keywords = "Adhesion, CrkL, EphB4, Fibronectin, c-Cbl",

author = "Hiroshi Sakamoto and Zhang, {Xiu Qin} and Souichi Suenobu and Kazuyuki Ohbo and Minetaro Ogawa and Toshio Suda",

note = "Funding Information: We wish to thank Dr. N. Komatsu and Dr. T. Kitamura for providing us UT-7/GM cells and an expression vector, pMX-IRES-GFP, respectively. This research is supported in part by Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.",

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doi = "10.1016/j.bbrc.2004.07.016",

language = "English",

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T1 - Cell adhesion to ephrinb2 is induced by EphB4 independently of its kinase activity

AU - Sakamoto, Hiroshi

AU - Zhang, Xiu Qin

AU - Suenobu, Souichi

AU - Ohbo, Kazuyuki

AU - Ogawa, Minetaro

AU - Suda, Toshio

N1 - Funding Information: We wish to thank Dr. N. Komatsu and Dr. T. Kitamura for providing us UT-7/GM cells and an expression vector, pMX-IRES-GFP, respectively. This research is supported in part by Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.

PY - 2004/8/27

Y1 - 2004/8/27

N2 - Cell to cell interaction in bone marrow is crucial for differentiation of hematopoietic cells. We have shown that EphB4 receptor is expressed in erythroid progenitor and its activation accelerates erythroid differentiation. To elucidate the role of EphB4 activation in erythropoiesis, we analyzed effects of EphB4 on cell adhesive pathways. Cell adhesion with the extension of filopodial pseudopod was observed by EphB4 activation. EphB4 activation also enhanced an effect of fibronectin-mediated adhesive pathway along with formation of the c-Cbl/CrkL complex. The tyrosine kinase activity of EphB4 was dispensable for those phenomena. These results suggest that activation of EphB4 participates in adhesive but not repulsive signals independently of its tyrosine kinase activity in hematopoietic cells.

AB - Cell to cell interaction in bone marrow is crucial for differentiation of hematopoietic cells. We have shown that EphB4 receptor is expressed in erythroid progenitor and its activation accelerates erythroid differentiation. To elucidate the role of EphB4 activation in erythropoiesis, we analyzed effects of EphB4 on cell adhesive pathways. Cell adhesion with the extension of filopodial pseudopod was observed by EphB4 activation. EphB4 activation also enhanced an effect of fibronectin-mediated adhesive pathway along with formation of the c-Cbl/CrkL complex. The tyrosine kinase activity of EphB4 was dispensable for those phenomena. These results suggest that activation of EphB4 participates in adhesive but not repulsive signals independently of its tyrosine kinase activity in hematopoietic cells.

KW - Adhesion

KW - CrkL

KW - EphB4

KW - Fibronectin

KW - c-Cbl

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EP - 687

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

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Cell adhesion to ephrinb2 is induced by EphB4 independently of its kinase activity

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