Cell-free extract based optimization of biomolecular circuits with droplet microfluidics

Yutaka Hori, Chaitanya Kantak, Richard M. Murray, Adam R. Abate

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.

本文言語English
ページ(範囲)3037-3042
ページ数6
ジャーナルLab on a Chip
17
18
DOI
出版ステータスPublished - 2017 9 21
外部発表はい

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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