Cell surface binding and activation of gelatinase A induced by expression of membrane-type-1-matrix metalloproteinase (MT1-MMP)

Hiroshi Sato, Takahisa Takino, Takeshi Kinoshita, Kazushi Imai, Yasunori Okada, William G. Stetler Stevenson, Motoharu Seiki

研究成果: Article査読

168 被引用数 (Scopus)

抄録

Gelatinase A is secreted as a proenzyme (progelatinase A) which is activated and bound on the surface of tumor and normal cells. We have reported that the expression of a membrane-type-1-matrix metalloproteinase (MT1-MMP) induces activation of progelatinase A. Here we demonstrate that the expression of MT1-MMP in COS-1 cells induces cell-surface binding of progelatinase A which is consequently processed to an intermediate form. Processing from the intermediate to the fully active form is dependent on the gelatinase A concentration. These results suggest that the cell-surface binding concentrates the gelatinase A intermediate form locally to allow autoproteolytic processing to the fully active form.

本文言語English
ページ(範囲)238-240
ページ数3
ジャーナルFEBS Letters
385
3
DOI
出版ステータスPublished - 1996 5 6

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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