Cellular dynamics of bovine aortic smooth muscle cells measured using MEMS force sensors

Takuya Tsukagoshi, Thanh Vinh Nguyen, Kayoko Hirayama Shoji, Hidetoshi Takahashi, Kiyoshi Matsumoto, Isao Shimoyama

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Adhesive cells perceive the mechanical properties of the substrates to which they adhere, adjusting their cellular mechanical forces according to their biological characteristics. This mechanical interaction subsequently affects the growth, locomotion, and differentiation of the cell. However, little is known about the detailed mechanism that underlies this interaction between adherent cells and substrates because dynamically measuring mechanical phenomena is difficult. Here, we utilize microelectromechamical systems force sensors that can measure cellular traction forces with high temporal resolution (∼2.5 μs) over long periods (∼3 h). We found that the cellular dynamics reflected physical phenomena with time scales from milliseconds to hours, which contradicts the idea that cellular motion is slow. A single focal adhesion (FA) generates an average force of 7 nN, which disappears in ms via the action of trypsin-ethylenediaminetetraacetic acid. The force-changing rate obtained from our measurements suggests that the time required for an FA to decompose was nearly proportional to the force acting on the FA.

本文言語English
論文番号145401
ジャーナルJournal of Physics D: Applied Physics
51
14
DOI
出版ステータスPublished - 2018 3 12
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 音響学および超音波学
  • 表面、皮膜および薄膜

フィンガープリント

「Cellular dynamics of bovine aortic smooth muscle cells measured using MEMS force sensors」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル