In this study, we purpose a centrifuge-based dynamic microarray system (CDM) for trapping micro-sized samples sequentially from only 10 μl of the samples. Our system enables us to make an array of microbeads only with a spin-coater. The air can be introduced to the microchannel to isolate and encapsulate the trapped beads by the specific speed rotation because the microbeads were fixed at the outlet of the trapping spots by the centrifugal force. We optimized rotation speed for trapping with the high trapping yield and successfully controlled sample/solution/air introduction. This device can also release the trapped beads by flowing liquid backward, which allows us to retrieve the microbeads and reuse the device. The presented technology marks an essential step towards a high efficient portable CDM for point-of-care use.