Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians

Yuri Miura, Noritaka Hashii, Hiroki Tsumoto, Daisuke Takakura, Yuki Ohta, Yukiko Abe, Yasumichi Arai, Nana Kawasaki, Nobuyoshi Hirose, Tamao Endo

研究成果: Article

13 引用 (Scopus)

抄録

An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto-and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-á, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.

元の言語English
記事番号e0142645
ジャーナルPLoS One
10
発行部数11
DOI
出版物ステータスPublished - 2015 11 1

Fingerprint

Glycosylation
glycosylation
blood proteins
Polysaccharides
Blood Proteins
polysaccharides
Aging of materials
inflammation
Glycomics
Inflammation
adiponectin
Adiponectin
Liquid chromatography
anti-inflammatory activity
interleukin-6
Liquid Chromatography
liquid chromatography
multivariate analysis
Mass spectrometry
Interleukin-6

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

これを引用

Miura, Y., Hashii, N., Tsumoto, H., Takakura, D., Ohta, Y., Abe, Y., ... Endo, T. (2015). Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians. PLoS One, 10(11), [e0142645]. https://doi.org/10.1371/journal.pone.0142645

Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians. / Miura, Yuri; Hashii, Noritaka; Tsumoto, Hiroki; Takakura, Daisuke; Ohta, Yuki; Abe, Yukiko; Arai, Yasumichi; Kawasaki, Nana; Hirose, Nobuyoshi; Endo, Tamao.

:: PLoS One, 巻 10, 番号 11, e0142645, 01.11.2015.

研究成果: Article

Miura, Y, Hashii, N, Tsumoto, H, Takakura, D, Ohta, Y, Abe, Y, Arai, Y, Kawasaki, N, Hirose, N & Endo, T 2015, 'Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians', PLoS One, 巻. 10, 番号 11, e0142645. https://doi.org/10.1371/journal.pone.0142645
Miura Y, Hashii N, Tsumoto H, Takakura D, Ohta Y, Abe Y その他. Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians. PLoS One. 2015 11 1;10(11). e0142645. https://doi.org/10.1371/journal.pone.0142645
Miura, Yuri ; Hashii, Noritaka ; Tsumoto, Hiroki ; Takakura, Daisuke ; Ohta, Yuki ; Abe, Yukiko ; Arai, Yasumichi ; Kawasaki, Nana ; Hirose, Nobuyoshi ; Endo, Tamao. / Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians. :: PLoS One. 2015 ; 巻 10, 番号 11.
@article{fdab16daf5a64a869fa1106fb4615ad9,
title = "Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians",
abstract = "An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto-and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-{\'a}, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.",
author = "Yuri Miura and Noritaka Hashii and Hiroki Tsumoto and Daisuke Takakura and Yuki Ohta and Yukiko Abe and Yasumichi Arai and Nana Kawasaki and Nobuyoshi Hirose and Tamao Endo",
year = "2015",
month = "11",
day = "1",
doi = "10.1371/journal.pone.0142645",
language = "English",
volume = "10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Change in N-glycosylation of plasma proteins in Japanese semisupercentenarians

AU - Miura, Yuri

AU - Hashii, Noritaka

AU - Tsumoto, Hiroki

AU - Takakura, Daisuke

AU - Ohta, Yuki

AU - Abe, Yukiko

AU - Arai, Yasumichi

AU - Kawasaki, Nana

AU - Hirose, Nobuyoshi

AU - Endo, Tamao

PY - 2015/11/1

Y1 - 2015/11/1

N2 - An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto-and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-á, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.

AB - An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto-and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-á, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.

UR - http://www.scopus.com/inward/record.url?scp=84955494519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84955494519&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0142645

DO - 10.1371/journal.pone.0142645

M3 - Article

C2 - 26559536

AN - SCOPUS:84955494519

VL - 10

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e0142645

ER -