Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells

Griselda Vallejo, Darío Maschi, Ana C. Mestre-Citrinovitz, Kazuhiro Aiba, Ricardo Maronna, Victor Yohai, Minoru S.H. Ko, Miguel Beato, Patricia Saragüeta

研究成果: Article査読

10 被引用数 (Scopus)

抄録

During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells.

本文言語English
ページ(範囲)127-137
ページ数11
ジャーナルJournal of Cellular Physiology
222
1
DOI
出版ステータスPublished - 2010 1
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 臨床生化学
  • 細胞生物学

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