Changes in the expression of epidermal differentiation markers at sites where cultured epithelial autografts were transplanted onto wounds from burn scar excision

Kuniko Kadoya, Satoshi Amano, Toshio Nishiyama, Shinji Inomata, Makoto Tsunenaga, Norio Kumagai, Kyoichi Matsuzaki

研究成果: Article

3 引用 (Scopus)

抄録

This study investigated the recovery process during which grafted cultured epithelium formed normal epidermis. The subjects were 18 patients whose burn scars were excised at a depth not exposing the fat layer and who subsequently received cultured epithelial autografts. A total of 24 samples were obtained from the grafted sites: 6 samples within 6weeks (stage 1), 5 samples after 6weeks and within 6months (stage 2), 6 samples after 6months and within 18months (stage 3) and 7 samples beyond 18months (stage 4) after transplantation. These samples were stained for monoclonal antibodies against filaggrin, transglutaminase (TG), cytokeratin 6 and involucrin. Their expressions were examined in the epidermis. The expression patterns were classified using a six-grade scale. The grades of filaggrin and TG were significantly higher at stage 3 and 4 compared with stage 1. There was a marginally significant increase in the grade of cytokeratin 6 at stage 3 and it was significantly higher at stage 4 compared with stage 1. These results showed that wound healing continued at a molecular level until the end of stage 3. The recovery of involucrin was delayed compared with that of other markers. TG and involucrin are thought to be regulated independently at the grafted sites.

元の言語English
ジャーナルInternational Wound Journal
DOI
出版物ステータスAccepted/In press - 2014
外部発表Yes

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Transglutaminases
Differentiation Antigens
Autografts
Keratin-6
Cicatrix
Epidermis
Wounds and Injuries
Wound Healing
Epithelium
Transplantation
Fats
Monoclonal Antibodies
involucrin
filaggrin

ASJC Scopus subject areas

  • Dermatology
  • Surgery

これを引用

Changes in the expression of epidermal differentiation markers at sites where cultured epithelial autografts were transplanted onto wounds from burn scar excision. / Kadoya, Kuniko; Amano, Satoshi; Nishiyama, Toshio; Inomata, Shinji; Tsunenaga, Makoto; Kumagai, Norio; Matsuzaki, Kyoichi.

:: International Wound Journal, 2014.

研究成果: Article

Kadoya, Kuniko ; Amano, Satoshi ; Nishiyama, Toshio ; Inomata, Shinji ; Tsunenaga, Makoto ; Kumagai, Norio ; Matsuzaki, Kyoichi. / Changes in the expression of epidermal differentiation markers at sites where cultured epithelial autografts were transplanted onto wounds from burn scar excision. :: International Wound Journal. 2014.
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AU - Amano, Satoshi

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AU - Inomata, Shinji

AU - Tsunenaga, Makoto

AU - Kumagai, Norio

AU - Matsuzaki, Kyoichi

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KW - Epidermal differentiation marker

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