Chapter 1 Monitoring Autophagy in Mammalian Cultured Cells through the Dynamics of LC3

Shunsuke Kimura, Naonobu Fujita, Takeshi Noda, Tamotsu Yoshimori

研究成果: Review article査読

203 被引用数 (Scopus)

抄録

In this chapter, we introduce several methods that rely on the analysis of LC3, a versatile marker protein of autophagic structures in mammalian cultured cells. The appearance of LC3-positive puncta is indicative of the induction of autophagy, and it is observed either by immunofluorescence or by GFP-based microscopy. The maturation process by which autophagosomes are converted into autolysosomes can be monitored by the GFP and RFP tandemly tagged LC3 (tfLC3) method. Lysosomal turnover of LC3 is a good index of the proceeding of autophagy and can be assessed by Western blotting. These methods will provide a relatively easy assessment of autophagy, and the details of the procedure will be described along with possible pitfalls.

本文言語English
ページ(範囲)1-12
ページ数12
ジャーナルMethods in Enzymology
451
C
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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