Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast

Masako Inuyama, Fumihiko Ushigome, Akiko Emoto, Noriko Koyabu, Shoji Satoh, Kiyomi Tsukimori, Hitoo Nakano, Hisakazu Ohtani, Yasufumi Sawada

研究成果: Article

16 引用 (Scopus)

抄録

The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane by using membrane vesicles isolated from human placenta. The uptake of L-[14C]lactic acid into basal membrane vesicles was Na+ independent, and an uphill transport was observed in the presence of a pH gradient ([H+]out > [H+]in). L-[14C]lactic acid uptake exhibited saturation kinetics with a Km value of 5.89 ± 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and α-cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4′-diisothiocyanostilbene-2,2′-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H+ dependent and Na+ independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, there were several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.

元の言語English
ジャーナルAmerican Journal of Physiology - Cell Physiology
283
発行部数3 52-3
出版物ステータスPublished - 2002 9
外部発表Yes

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Trophoblasts
Lactic Acid
Membranes
Brushes
Microvilli
Proton-Motive Force
Monocarboxylic Acid Transporters
4-Chloromercuribenzenesulfonate
Phloretin
Dicarboxylic Acids
Active Biological Transport
Placenta
Kinetics

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

これを引用

Inuyama, M., Ushigome, F., Emoto, A., Koyabu, N., Satoh, S., Tsukimori, K., ... Sawada, Y. (2002). Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast. American Journal of Physiology - Cell Physiology, 283(3 52-3).

Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast. / Inuyama, Masako; Ushigome, Fumihiko; Emoto, Akiko; Koyabu, Noriko; Satoh, Shoji; Tsukimori, Kiyomi; Nakano, Hitoo; Ohtani, Hisakazu; Sawada, Yasufumi.

:: American Journal of Physiology - Cell Physiology, 巻 283, 番号 3 52-3, 09.2002.

研究成果: Article

Inuyama, M, Ushigome, F, Emoto, A, Koyabu, N, Satoh, S, Tsukimori, K, Nakano, H, Ohtani, H & Sawada, Y 2002, 'Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast', American Journal of Physiology - Cell Physiology, 巻. 283, 番号 3 52-3.
Inuyama, Masako ; Ushigome, Fumihiko ; Emoto, Akiko ; Koyabu, Noriko ; Satoh, Shoji ; Tsukimori, Kiyomi ; Nakano, Hitoo ; Ohtani, Hisakazu ; Sawada, Yasufumi. / Characteristics of L-lactic acid transport in basal membrane vesicles of human placental syncytiotrophoblast. :: American Journal of Physiology - Cell Physiology. 2002 ; 巻 283, 番号 3 52-3.
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AU - Ushigome, Fumihiko

AU - Emoto, Akiko

AU - Koyabu, Noriko

AU - Satoh, Shoji

AU - Tsukimori, Kiyomi

AU - Nakano, Hitoo

AU - Ohtani, Hisakazu

AU - Sawada, Yasufumi

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N2 - The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane by using membrane vesicles isolated from human placenta. The uptake of L-[14C]lactic acid into basal membrane vesicles was Na+ independent, and an uphill transport was observed in the presence of a pH gradient ([H+]out > [H+]in). L-[14C]lactic acid uptake exhibited saturation kinetics with a Km value of 5.89 ± 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and α-cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4′-diisothiocyanostilbene-2,2′-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H+ dependent and Na+ independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, there were several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.

AB - The characteristics of L-lactic acid transport across the trophoblast basal membrane were investigated and compared with those across the brush-border membrane by using membrane vesicles isolated from human placenta. The uptake of L-[14C]lactic acid into basal membrane vesicles was Na+ independent, and an uphill transport was observed in the presence of a pH gradient ([H+]out > [H+]in). L-[14C]lactic acid uptake exhibited saturation kinetics with a Km value of 5.89 ± 0.68 mM in the presence of a pH gradient. p-Chloromercuribenzenesulfonate and α-cyano-4-hydroxycinnamate inhibited the initial uptake, whereas phloretin or 4,4′-diisothiocyanostilbene-2,2′-disulfonate did not. Mono- and dicarboxylic acids suppressed the initial uptake. In conclusion, L-lactic acid transport in the basal membrane is H+ dependent and Na+ independent, as is also the case for the brush-border membrane transport, and its characteristics resemble those of monocarboxylic acid transporters. However, there were several differences in the effects of inhibitors between basal and brush-border membrane vesicles, suggesting that the transporter(s) involved in L-lactic acid transport in the basal membrane of placental trophoblast may differ from those in the brush-border membrane.

KW - Human placenta

KW - Monocarboxylic acid

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