We proposed novel depth-controlled photodynamie therapy (PDT) by varying pulsed laser irradiation condition. We focus on photoeytotoxicity suppression under high peak power pulsed irradiation to apply this phenomenon to surface intact therapy to preserve healthy wall of a hollow organ. The relation between laser irradiation condition and cell lethality of rat prostate cancer cell with PDT using practical photosensitizers, mono-L-aspartyl chlorine e6 (ME2906) and Photofrin® was investigated. We found cell lethality suppression from 63 % to 20 % when the irradiated pulsed peak power density ranged from 0.2 to 1.4 mW/cm with ME2906 mediated PDT. There was no significant photoeytotoxicity suppression in case of Photofrin® mediated PDT. Singlet oxygen luminescence from the two different kinds of photosensitizer solution was measured. The pulse peak power dependence of singlet oxygen luminescence intensity correlated with the photoeytotoxicity. We think the photoeytotoxicity suppression with high peak power pulsed irradiation with ME2906 might be useful for the therapeutic depth controlled PDT without damage on the surface of a hollow organ.