@article{a491d50d66d74c5f91f96d6362b78b46,
title = "Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells",
abstract = "We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.",
keywords = "ABC transporter, ABCB1, Cell surface marker, Neural stem cell",
author = "Islam, {Mohammed Omedul} and Yonehiro Kanemura and Jesmin Tajria and Hideki Mori and Satoshi Kobayashi and Tomoko Shofuda and Jun Miyake and Masayuki Hara and Mami Yamasaki and Hideyuki Okano",
note = "Funding Information: We thank Dr. Chiaki Ban at Osaka National Hospital for providing the human fetal tissues. This study was supported in part by the Millennium Project from the Ministry of Economy, Trade and Industry of Japan, and by the Cooperative Link of Unique Science and Technology for Economy Revitalization (CLUSTER) project, the Project for Realization of Regenerative Medicine, the research field for the Development of Stem Cell Therapy from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",
year = "2005",
month = jul,
day = "27",
doi = "10.1016/j.febslet.2005.05.019",
language = "English",
volume = "579",
pages = "3473--3480",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "17",
}