Characterization of DNA hypermethylation in two cases of peritoneal mesothelioma.

Ryota Hama, Yoshiyuki Watanabe, Kanako Shinada, Yosuke Yamada, Yuka Ogata, Yoshihito Yoshida, Tomohiro Tamura, Tetsuya Hiraishi, Ritsuko Oikawa, J. Sakurai, Tadateru Maehata, Hirotaka Koizumi, Fumio Itoh

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Malignant mesothelioma (MM) is a rare disease with a poor prognosis. Pleural mesothelioma, which is the most common type of MM, is considered to be caused by asbestos exposure and is increasing in incidence, with about 15,000 new cases diagnosed worldwide annually. On the other hand, peritoneal mesothelioma is a very rare type of MM; thus, its pathogenesis is even less understood than pleural mesothelioma. Recent research on the pathogenesis of malignant pleural mesothelioma has indicated that both epigenetic and genetic alterations contribute to tumorigenesis. Here, we hypothesize that peritoneal mesothelioma also has an epigenetic alteration in the same genes (Kazal-type serine peptidase inhibitor domain 1 (KAZALD1), transmembrane protein 30B (TMEM30B), and mitogen-activated protein kinase 13 (MAPK13)). Our goal is to identify DNA methylation of these three candidate genes in two peritoneal mesothelioma cases. Laser capture microdissection was used to separate diseased sections of formalin-fixed paraffin-embedded samples from one surgically resected tissue (epithelial type) and one autopsy tissue (sarcomatous type). Genomic DNA was subsequently extracted by the standard phenol chloroform method. The DNA was then treated with sodium bisulphite, and pyrosequencing analysis was used to quantitatively analyze the methylation of candidate genes reported to be hypermethylated in malignant pleural mesothelioma (KAZALD1, TMEM30B, and MAPK13). TMEM30B and MAPK13 were not methylated in either case. However, KAZALD1 was highly methylated in sarcomatoid-type peritoneal mesothelioma. We first report that the KAZALD1 gene was hypermethylated in sarcomatoid-type malignant peritoneal mesothelioma.

本文言語English
ページ(範囲)2031-2040
ページ数10
ジャーナルTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
33
6
DOI
出版ステータスPublished - 2012 12

ASJC Scopus subject areas

  • 癌研究

フィンガープリント

「Characterization of DNA hypermethylation in two cases of peritoneal mesothelioma.」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル