Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.

Yuki Haraguchi; Kanako Kuwasako; Yutaka Muto; Yoshitaka Bessho; Madoka Nishimoto; Shigeyuki Yokoyama; Akio Kanai; Gota Kawai; Taiichi Sakamoto

doi:10.1093/nass/nrp133

Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.

Yuki Haraguchi, Kanako Kuwasako, Yutaka Muto, Yoshitaka Bessho, Madoka Nishimoto, Shigeyuki Yokoyama, Akio Kanai, Gota Kawai, Taiichi Sakamoto

研究成果: Article › 査読

1 被引用数 (Scopus)

抄録

SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.

本文言語	English
ページ（範囲）	265-266
ページ数	2
ジャーナル	Nucleic acids symposium series (2004)
号	53
DOI	https://doi.org/10.1093/nass/nrp133
出版ステータス	Published - 2009
外部発表	はい

ASJC Scopus subject areas

医学（全般）

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10.1093/nass/nrp133

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AU - Kuwasako, Kanako

AU - Muto, Yutaka

AU - Bessho, Yoshitaka

AU - Nishimoto, Madoka

AU - Yokoyama, Shigeyuki

AU - Kanai, Akio

AU - Kawai, Gota

AU - Sakamoto, Taiichi

PY - 2009

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AB - SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.

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Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.

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