Chemical synthesis of peptidoglycan fragments for elucidation of the immunostimulating mechanism

Yukari Fujimoto, Seiichi Inamura, Akiko Kawasaki, Zenyu Shiokawa, Atsushi Shimoyama, Takashi Hashimoto, Shoichi Kusumoto, Koichi Fukase

研究成果: Review article査読

17 被引用数 (Scopus)

抄録

Partial structures of peptidoglycan were chemically synthesized for elucidation of their precise biological activities. By using an efficient synthetic strategy, mono-, di-, tetra- and octasaccharide fragments of peptidoglycan were synthesized in good yields. The biological activity of synthetic fragments of peptidoglycan was evaluated by induction of TNF-α from human monocytes, and TLR2 and NOD2 dependencies by using transfected HEK293 cells, respectively. We revealed that TLR2 was not stimulated by the series of synthetic peptidoglycan partial structures, whereas NOD2 recognizes the partial structures containing the MDP moiety. We also synthesized potent NOD1 agonists, which showed several hundred-fold stronger activity than γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP). Interaction of PGRPs with synthetic peptidoglycan fragments is also described.

本文言語English
ページ(範囲)189-196
ページ数8
ジャーナルJournal of Endotoxin Research
13
3
DOI
出版ステータスPublished - 2007 6月
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • 分子生物学
  • 細胞生物学
  • 感染症

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