@article{fe4eab0037aa4d12a78bb15cb91cbecd,
title = "Chemoenzymatic semisynthesis of caffeic acid β-phenethyl ester, an antioxidative component in propolis, from raw coffee bean extract",
abstract = "Caffeic acid {\ss}-phenethyl ester (CAPE), an antioxidative bioactive catechol isolated from propolis, was semisynthesized from chlorogenic acid and related compounds in an extract of raw (unroasted) Robusta coffee (Coffea canephora) beans in 5 steps and a total yield of 31%. Oxidative degradation of the intermediates and target molecule was prevented by alkaline hydrolysis of the chlorogenic acids in the presence of sodium dithionite (Na2S2O4) and deprotection of the catecholic diacetate precursor by Candida antarctica lipase B-mediated transesterification as the final step.",
keywords = "caffeic acid, catechol, chlorogenic acid, cinnamate ester, lipase-catalyzed deacetylation",
author = "Riichi Hashimoto and Hiyori Iai and Rie Fujita and Kengo Hanaya and Shuhei Higashibayashi and Hiroyoshi Inoue and Takeshi Sugai",
note = "Funding Information: This work was supported by JSPS KAKENHI (19K05849) for T.S. and is gratefully acknowledged. This work was also supported by Global Science Campus, Japan Science and Technology Agency for H.I. (Inoue) and H.I. (Iai) thanks for the participation of this program. Publisher Copyright: {\textcopyright} The Author(s) 2021.",
year = "2021",
month = mar,
day = "1",
doi = "10.1093/bbb/zbaa077",
language = "English",
volume = "85",
pages = "476--480",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "3",
}