Chromatographic separation and identification of human urine components

Y. Nagata, Y. Matsushima, H. Ugai, M. Kikuchi, N. Takai

研究成果: Article査読

抄録

Ultraviolet-absorbing components of human urine were separated by HPLC with a large scale column (250 × 28 mm I.D. stainless-steel) containing a macroreticular type strongly basic anion-exchange resin. The sample was eluted with a linear gradient of 0.006 M to 6.0 M ammonium acetate buffer (pH 4.40) and different fractions were collected. The identification of one compound using ultraviolet, infrared and mass spectral data is illustrated; it was found to be uric acid.

本文言語English
ページ(範囲)317-318
ページ数2
ジャーナルChromatographia
24
1
DOI
出版ステータスPublished - 1987 12月

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 臨床生化学
  • 有機化学

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