TY - JOUR
T1 - Chromosomal mapping of a family of human glutamine synthetase genes
T2 - Functional gene (GLUL) on 1q25, pseudogene (GLULP) on 9p13, and three related genes (GLULL1, GLULL2, GLULL3) on 5q33, 11p15, and 11q24
AU - Wang, Yimin
AU - Kudoh, Jun
AU - Kubota, Ryo
AU - Asakawa, Shuichi
AU - Minoshima, Shinsei
AU - Shimizu, Nobuyoshi
PY - 1996/10/15
Y1 - 1996/10/15
N2 - Glutamine synthetase (GS) is a ubiquitous enzyme that catalyzes the ATP- dependent conversion of glutamate to glutamine using ammonia as the nitrogen source. Using human GS cDNA as a probe, a bacterial artificial chromosome (BAC) library consisting of twofold coverage of the human genome was screened, and 18 clones were obtained. The restriction analysis of the human insert DNAs provided a basis to divide these 18 BAC clones into five groups, suggesting the existence of a GS gene family in the human genome. PCR analysis using three sets of primers designed from the reported sequences for GS cDNA and a processed pseudogene identified the corresponding BAC clones. Fluorescence in situ hybridization analysis revealed the chromosomal localization of these five genes: the GS gene to 1q25 (GLUL), the processed pseudogene to 9p13 (GLULP), and three related genes to 5q33 (GLULL1), 11p15 (GLULL2), and 11q24 (GLULL3), respectively.
AB - Glutamine synthetase (GS) is a ubiquitous enzyme that catalyzes the ATP- dependent conversion of glutamate to glutamine using ammonia as the nitrogen source. Using human GS cDNA as a probe, a bacterial artificial chromosome (BAC) library consisting of twofold coverage of the human genome was screened, and 18 clones were obtained. The restriction analysis of the human insert DNAs provided a basis to divide these 18 BAC clones into five groups, suggesting the existence of a GS gene family in the human genome. PCR analysis using three sets of primers designed from the reported sequences for GS cDNA and a processed pseudogene identified the corresponding BAC clones. Fluorescence in situ hybridization analysis revealed the chromosomal localization of these five genes: the GS gene to 1q25 (GLUL), the processed pseudogene to 9p13 (GLULP), and three related genes to 5q33 (GLULL1), 11p15 (GLULL2), and 11q24 (GLULL3), respectively.
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U2 - 10.1006/geno.1996.0542
DO - 10.1006/geno.1996.0542
M3 - Article
C2 - 8921392
AN - SCOPUS:0030588067
VL - 37
SP - 195
EP - 199
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 2
ER -