Chromosome spread analyses of meiotic sex chromosome inactivation

Kris G. Alavattam, Hironori Abe, Akihiko Sakashita, Satoshi H. Namekawa

研究成果: Chapter

6 被引用数 (Scopus)

抄録

A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ113-129
ページ数17
DOI
出版ステータスPublished - 2018
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1861
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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