Circulating anti-double-stranded DNA antibody-secreting cells in patients with systemic lupus erythematosus: A novel biomarker for disease activity

H. Hanaoka, Y. Okazaki, T. Satoh, Yuko Kaneko, Hidekata Yasuoka, N. Seta, M. Kuwana

研究成果: Article

12 引用 (Scopus)

抄録

Antibodies against double-stranded DNA (dsDNA) are widely used to diagnose systemic lupus erythematosus (SLE) and evaluate its activity in patients. This study was undertaken to examine the clinical utility of circulating anti-dsDNA antibody-secreting cells for evaluating SLE patients. Anti-dsDNA antibody-secreting cells quantified using an enzyme-linked immunospot assay were detected in the spleen, bone marrow and peripheral blood from MRL/lpr but not in control BALB/c mice. Circulating anti-dsDNA antibody-secreting cells were detected in 29 (22%) of 130 patients with SLE, but in none of 49 with non-SLE connective-tissue disease or 18 healthy controls. The presence of circulating anti-dsDNA antibody-secreting cells was associated with persistent proteinuria, high SLE disease activity index and systemic lupus activity measures, and a high serum anti-dsDNA antibody titre measured with an enzyme-linked immunosorbent assay. The positive predictive value for active disease was 48% for circulating anti-dsDNA antibody-secreting cells versus 17% for serum anti-dsDNA antibodies. A prospective cohort of patients with circulating anti-dsDNA antibodies and inactive SLE showed that the cumulative disease flare-free rate was significantly lower in patients with than without circulating anti-dsDNA antibody-secreting cells (p<0.001). Circulating anti-dsDNA antibody-secreting cells are a useful biomarker for assessing disease activity in SLE patients.

元の言語English
ページ(範囲)1284-1293
ページ数10
ジャーナルLupus
21
発行部数12
DOI
出版物ステータスPublished - 2012 10

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Antibody-Producing Cells
Systemic Lupus Erythematosus
Biomarkers
DNA
Antibodies
Enzyme-Linked Immunospot Assay
Connective Tissue Diseases
Serum
Proteinuria
Spleen
Bone Marrow
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Rheumatology

これを引用

Circulating anti-double-stranded DNA antibody-secreting cells in patients with systemic lupus erythematosus : A novel biomarker for disease activity. / Hanaoka, H.; Okazaki, Y.; Satoh, T.; Kaneko, Yuko; Yasuoka, Hidekata; Seta, N.; Kuwana, M.

:: Lupus, 巻 21, 番号 12, 10.2012, p. 1284-1293.

研究成果: Article

Hanaoka, H. ; Okazaki, Y. ; Satoh, T. ; Kaneko, Yuko ; Yasuoka, Hidekata ; Seta, N. ; Kuwana, M. / Circulating anti-double-stranded DNA antibody-secreting cells in patients with systemic lupus erythematosus : A novel biomarker for disease activity. :: Lupus. 2012 ; 巻 21, 番号 12. pp. 1284-1293.
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