Circulating autoantibodies against purified colonic mucin in ulcerative colitis

Hiromasa Takaishi, Susumu Ohara, Kyoko Hotta, Tomoharu Yajima, Takanori Kanai, Nagamu Inoue, Yasushi Iwao, Mamoru Watanabe, Hiromasa Ishii, Toshifumi Hibi

研究成果: Article

16 引用 (Scopus)

抄録

To clarify the role of colonic mucin in the autoimmune process of ulcerative colitis, circulating antibodies against human colonic mucin were investigated. Purified colonic mucin, obtained from human colonic mucosa by gel filtration, using a Bio-Gel A-1.5-m column and CsCl equilibrium density gradient, was divided into soluble mucin (S-mucin) secreted extracellularly and membranous mucin (M-mucin) binding to cell membrane. Sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blotting analysis showed that antibodies in the serum samples of some patients with ulcerative colitis recognized purified S- and M-mucin of >180-kD. By enzyme-linked immunosorbent assay (ELISA), anti-mucin antibodies were detected in 11 of 60 patients with ulcerative colitis (18). In contrast, the antibodies were not detected in 22 patients with Crohn's disease. The titers of anti-mucin antibodies against S-mucin and M-mucin were not different in each patient. By ELISA using mucin in which the sugar chains were destroyed by neuraminidase or NaIO4 treatment, it was demonstrated that anti-mucin antibodies recognized the epitopes of either the sugar chain or the core protein exposed through destruction of the sugar chains. We then investigated the relationship between anti-mucin antibodies and the patients' clinical features. Anti-mucin antibodies were detected in 6 of 15 patients with chronic continuous type ulcerative colitis (40) and in 5 of 26 patients with relapsing-remitting type (19), but there was no anti-mucin antibody-positive serum in patients who had had only one attack without any relapse. These results suggest that anti-mucin antibodies could be a disease marker for ulcerative colitis and that immunological abnormalities in colonic mucin contribute to the persistence of colonic mucosal inflammation.

元の言語English
ページ(範囲)20-27
ページ数8
ジャーナルJournal of Gastroenterology
35
発行部数1
出版物ステータスPublished - 2000 1

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Mucins
Ulcerative Colitis
Autoantibodies
Anti-Idiotypic Antibodies
Antibodies
Enzyme-Linked Immunosorbent Assay
Neuraminidase
Serum
Crohn Disease

ASJC Scopus subject areas

  • Gastroenterology

これを引用

Circulating autoantibodies against purified colonic mucin in ulcerative colitis. / Takaishi, Hiromasa; Ohara, Susumu; Hotta, Kyoko; Yajima, Tomoharu; Kanai, Takanori; Inoue, Nagamu; Iwao, Yasushi; Watanabe, Mamoru; Ishii, Hiromasa; Hibi, Toshifumi.

:: Journal of Gastroenterology, 巻 35, 番号 1, 01.2000, p. 20-27.

研究成果: Article

Takaishi, H, Ohara, S, Hotta, K, Yajima, T, Kanai, T, Inoue, N, Iwao, Y, Watanabe, M, Ishii, H & Hibi, T 2000, 'Circulating autoantibodies against purified colonic mucin in ulcerative colitis', Journal of Gastroenterology, 巻. 35, 番号 1, pp. 20-27.
Takaishi, Hiromasa ; Ohara, Susumu ; Hotta, Kyoko ; Yajima, Tomoharu ; Kanai, Takanori ; Inoue, Nagamu ; Iwao, Yasushi ; Watanabe, Mamoru ; Ishii, Hiromasa ; Hibi, Toshifumi. / Circulating autoantibodies against purified colonic mucin in ulcerative colitis. :: Journal of Gastroenterology. 2000 ; 巻 35, 番号 1. pp. 20-27.
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abstract = "To clarify the role of colonic mucin in the autoimmune process of ulcerative colitis, circulating antibodies against human colonic mucin were investigated. Purified colonic mucin, obtained from human colonic mucosa by gel filtration, using a Bio-Gel A-1.5-m column and CsCl equilibrium density gradient, was divided into soluble mucin (S-mucin) secreted extracellularly and membranous mucin (M-mucin) binding to cell membrane. Sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blotting analysis showed that antibodies in the serum samples of some patients with ulcerative colitis recognized purified S- and M-mucin of >180-kD. By enzyme-linked immunosorbent assay (ELISA), anti-mucin antibodies were detected in 11 of 60 patients with ulcerative colitis (18). In contrast, the antibodies were not detected in 22 patients with Crohn's disease. The titers of anti-mucin antibodies against S-mucin and M-mucin were not different in each patient. By ELISA using mucin in which the sugar chains were destroyed by neuraminidase or NaIO4 treatment, it was demonstrated that anti-mucin antibodies recognized the epitopes of either the sugar chain or the core protein exposed through destruction of the sugar chains. We then investigated the relationship between anti-mucin antibodies and the patients' clinical features. Anti-mucin antibodies were detected in 6 of 15 patients with chronic continuous type ulcerative colitis (40) and in 5 of 26 patients with relapsing-remitting type (19), but there was no anti-mucin antibody-positive serum in patients who had had only one attack without any relapse. These results suggest that anti-mucin antibodies could be a disease marker for ulcerative colitis and that immunological abnormalities in colonic mucin contribute to the persistence of colonic mucosal inflammation.",
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AU - Takaishi, Hiromasa

AU - Ohara, Susumu

AU - Hotta, Kyoko

AU - Yajima, Tomoharu

AU - Kanai, Takanori

AU - Inoue, Nagamu

AU - Iwao, Yasushi

AU - Watanabe, Mamoru

AU - Ishii, Hiromasa

AU - Hibi, Toshifumi

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N2 - To clarify the role of colonic mucin in the autoimmune process of ulcerative colitis, circulating antibodies against human colonic mucin were investigated. Purified colonic mucin, obtained from human colonic mucosa by gel filtration, using a Bio-Gel A-1.5-m column and CsCl equilibrium density gradient, was divided into soluble mucin (S-mucin) secreted extracellularly and membranous mucin (M-mucin) binding to cell membrane. Sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blotting analysis showed that antibodies in the serum samples of some patients with ulcerative colitis recognized purified S- and M-mucin of >180-kD. By enzyme-linked immunosorbent assay (ELISA), anti-mucin antibodies were detected in 11 of 60 patients with ulcerative colitis (18). In contrast, the antibodies were not detected in 22 patients with Crohn's disease. The titers of anti-mucin antibodies against S-mucin and M-mucin were not different in each patient. By ELISA using mucin in which the sugar chains were destroyed by neuraminidase or NaIO4 treatment, it was demonstrated that anti-mucin antibodies recognized the epitopes of either the sugar chain or the core protein exposed through destruction of the sugar chains. We then investigated the relationship between anti-mucin antibodies and the patients' clinical features. Anti-mucin antibodies were detected in 6 of 15 patients with chronic continuous type ulcerative colitis (40) and in 5 of 26 patients with relapsing-remitting type (19), but there was no anti-mucin antibody-positive serum in patients who had had only one attack without any relapse. These results suggest that anti-mucin antibodies could be a disease marker for ulcerative colitis and that immunological abnormalities in colonic mucin contribute to the persistence of colonic mucosal inflammation.

AB - To clarify the role of colonic mucin in the autoimmune process of ulcerative colitis, circulating antibodies against human colonic mucin were investigated. Purified colonic mucin, obtained from human colonic mucosa by gel filtration, using a Bio-Gel A-1.5-m column and CsCl equilibrium density gradient, was divided into soluble mucin (S-mucin) secreted extracellularly and membranous mucin (M-mucin) binding to cell membrane. Sodium dodecylsulfate polyacrylamide gel electrophoresis and Western blotting analysis showed that antibodies in the serum samples of some patients with ulcerative colitis recognized purified S- and M-mucin of >180-kD. By enzyme-linked immunosorbent assay (ELISA), anti-mucin antibodies were detected in 11 of 60 patients with ulcerative colitis (18). In contrast, the antibodies were not detected in 22 patients with Crohn's disease. The titers of anti-mucin antibodies against S-mucin and M-mucin were not different in each patient. By ELISA using mucin in which the sugar chains were destroyed by neuraminidase or NaIO4 treatment, it was demonstrated that anti-mucin antibodies recognized the epitopes of either the sugar chain or the core protein exposed through destruction of the sugar chains. We then investigated the relationship between anti-mucin antibodies and the patients' clinical features. Anti-mucin antibodies were detected in 6 of 15 patients with chronic continuous type ulcerative colitis (40) and in 5 of 26 patients with relapsing-remitting type (19), but there was no anti-mucin antibody-positive serum in patients who had had only one attack without any relapse. These results suggest that anti-mucin antibodies could be a disease marker for ulcerative colitis and that immunological abnormalities in colonic mucin contribute to the persistence of colonic mucosal inflammation.

KW - Anti-mucin antibodies

KW - Crohn's disease

KW - ELISA

KW - Mucin

KW - Ulcerative colitis

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