Cloning and characterization of a novel NMDA receptor subunit NR3B: A dominant subunit that reduces calcium permeability

Keiko Matsuda, Yoshinori Kamiya, Shinji Matsuda, Michisuke Yuzaki

研究成果: Article

149 引用 (Scopus)

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We report the cloning and characterization of a novel NMDA receptor subunit cDNA, which encodes a predicted polypeptide of 1003 amino acids. Phylogenic analysis indicates that this new subunit is most closely related to NR3A. Therefore, we term it NR3B. Important functional domains of glutamate receptors, such as the ligand-binding domain, the channel pore, and the channel gate, are conserved in NR3B. NR3B mRNA was expressed highly in pons, midbrain, medulla, and the spinal cord, but at low levels in the forebrain and the cerebellum. Although NR3A mRNA expression decreases sharply after the second postnatal weeks, NR3B mRNA expression levels in whole brain were constant during postnatal development and into adult. Coimmunoprecipitation analysis showed that NR3B could form NMDA receptor complex with NR1a and NR2A subunits in heterologous cells. Although expression of NR3B alone did not reconstitute functional NMDA receptors, coexpression of NR3B reduced the Ca2+ permeability of glutamate-induced currents in cells expressing NR1a and NR2A. These results indicate that NR3B is a dominant modulatory subunit that can modify the function of NMDA receptors. Since high Ca2+ permeability of NMDA receptors is thought to be a key feature for NMDA receptors to play critical roles in neurodevelopment, synaptic plasticity, and neuronal death, NR3B may contribute to the regulation of these physiological and pathological processes.

元の言語English
ページ(範囲)43-52
ページ数10
ジャーナルMolecular Brain Research
100
発行部数1-2
DOI
出版物ステータスPublished - 2002 4 30
外部発表Yes

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ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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