Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells

Masanobu Kawasaki, Shinichi Uchida, Toshiaki Monkawa, Atsushi Miyawaki, Katsuhiko Mikoshiba, Fumiaki Marumo, Sei Sasaki

研究成果: Article査読

215 被引用数 (Scopus)

抄録

cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3 mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.

本文言語English
ページ(範囲)597-604
ページ数8
ジャーナルNeuron
12
3
DOI
出版ステータスPublished - 1994 3月

ASJC Scopus subject areas

  • 神経科学(全般)

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