Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver

Shigeru B.H. Ko, Shinichi Uchida, Satoru Naruse, Michio Kuwahara, Kenichi Ishibashi, Fumiaki Marumo, Tetsuo Hayakawa, Sei Sasaki

研究成果: Article

54 引用 (Scopus)

抜粋

A new aquaporin was isolated from rat liver based on homology to known aquaporins. A 1408 bp cDNA was sequenced (designated rAQP9L) with a 885 bp open reading frame encoding a 295 amino acid hydrophobic protein. rAQP9L has the greatest amino-acid sequence identity with human AQP9 (75%) and a less homology with AQP3 (49%) and AQP7 (47%). Northern blot analysis indicated a 1.4-kb transcript expressed strongly in liver>testis>brain=lung. Expression of rAQP9L cRNA in Xenopus oocytes increased osmotic water permeability by 6-folds which was inhibited by 0.3 mM mercury chloride by 42%. rAQP9L also facilitated glycerol and urea transport by 2- and 5-folds, respectively. The large discrepancy of tissue distribution between hAQP9 and rAQP9L suggest that rAQP9L is a new aquaporin, which is involved in transport of urea as well as water in liver.

元の言語English
ページ(範囲)309-318
ページ数10
ジャーナルBiochemistry and Molecular Biology International
47
発行部数2
出版物ステータスPublished - 1999 2 1
外部発表Yes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

これを引用

Ko, S. B. H., Uchida, S., Naruse, S., Kuwahara, M., Ishibashi, K., Marumo, F., Hayakawa, T., & Sasaki, S. (1999). Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver. Biochemistry and Molecular Biology International, 47(2), 309-318.