Cloning, expression, and characterization of cytosolic sulfotransferase isozymes from Drosophila melanogaster

Kenji Hattori, Noriko Motohashi, Izumi Kobayashi, Takeshi Tohya, Mai Oikawa, Hiro Omi Tamura

研究成果: Article査読

15 被引用数 (Scopus)

抄録

We have identified four cytosolic sulfotransferase (SULT) homologs in the genome database of Drosophila melanogaster, and have designated these genes dmST1-4. Each of these four isozymes was subsequently classified into a different and novel gene family, as determined by the low amino acid sequence homology (less than 40%) between them, and also toward their vertebrate homologs. The transcripts for these four SULT homologs were detectable at all developmental stages in D. melanogaster. In addition, three of these isozymes, the exception being dmST2, were successfully expressed and purified in Escherichia coli. These recombinant dmST1, 3, and 4 products showed high sulfating activity toward phenolic compounds such as vanillin and 4-nitrophenol, but showed no activity toward typical endogenous substrates such as tyramine and serotonin. DmST4 and dmST3 showed the lowest and highest Km values for 3′-phosphoadenosine-5′-phosphosulfate (PAPS) respectively. DmST4 also showed low but not negligible activity toward 20-hydroxyecdysone.

本文言語English
ページ(範囲)540-547
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
72
2
DOI
出版ステータスPublished - 2008 3月 13
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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