Cloning of a hsp70-related gene expressed in mouse spermatids

Midori Matsumoto, Hirokazu Fujimoto

研究成果: Article査読

106 被引用数 (Scopus)

抄録

A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.

本文言語English
ページ(範囲)43-49
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
166
1
DOI
出版ステータスPublished - 1990 1 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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