Coherent X-ray DIFFRACTION IMAGINg of Cyanidioschyzon merolae

Yuki Sekiguchi, Amane Kobayashi, Yuki Takayama, Mao Oide, Asahi Fukuda, Takahiro Yamamoto, Koji Okajima, Tomotaka Oroguchi, Takeshi Hirakawa, Yayoi Inui, Sachihiro Matsunaga, Masaki Yamamoto, Masayoshi Nakasako

研究成果: Chapter

抄録

Coherent X-ray diffraction imaging (CXDI) is a lensless imaging technique for visualizing the structures of noncrystalline particles at a resolution of several tens of nanometers. The targets are particles with dimensions in the sub-micrometer to micrometer range. We carried out CXDI experiments at 66 K to investigate the internal structures of a whole frozen-hydrated cell and a chloroplast of Cyanidioschyzon merolae and a cyanobacteria cell by using coherent X-ray light sources, such as synchrotron and X-ray free-electron laser facilities. Owing to the short wavelength of the X-rays used (0.225 nm), the absorption and multiple scattering of X-rays inside the specimens were negligible. Diffraction patterns from each specimen particle adsorbed onto a thin membrane were collected at resolutions better than 50 μm -1 in reciprocal space. Therefore, structures of specimen particles can be illustrated at a resolution higher than 200 nm in real space. The most probable electron density map was retrieved from each diffraction pattern. Theinternal structures of the specimens are described here, particularly the structural correlation of the chloroplast of C. merolae and the cyanobacteria cell. Based on the experimental results, we discuss the feasibility of CXDI in the structural analyses of biological cells and cellular organelles.

本文言語English
ホスト出版物のタイトルCyanidioschyzon merolae
ホスト出版物のサブタイトルA New Model Eukaryote for Cell and Organelle Biology
出版社Springer Singapore
ページ153-173
ページ数21
ISBN(電子版)9789811061011
ISBN(印刷版)9789811061004
DOI
出版ステータスPublished - 2018 3 8

ASJC Scopus subject areas

  • 免疫学および微生物学(全般)
  • 農業および生物科学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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