Combined Gene Expression and Chromatin Immunoprecipitation From a Single Mouse Hippocampus

Jean Christophe Helbling, Kenichiro Kinouchi, Pierre Trifilieff, Paolo Sassone-Corsi, Marie Pierre Moisan

研究成果: Article査読

1 被引用数 (Scopus)

抄録

All neuronal cells hold the same genetic information but vary by their structural and functional plasticity depending on the brain area and environmental influences. Such variability involves specific gene regulation, which is driven by transcription factors (TFs). In the field of neuroscience, epigenetics is the main mechanism that has been investigated to understand the dynamic modulation of gene expression by behavioral responses, stress responses, memory processes, etc. Nowadays, gene expression analyzed by real-time quantitative PCR and TF binding estimated by chromatin immunoprecipitation (ChIP) enables one to dissect this regulation. Because of the wide range of transgenic models, as well as cost-effective aspects, mouse models are widely used neuroscience. Thus, we have set up a protocol that allows extraction of both RNA for gene expression analysis and chromatin for ChIP experiment from a single mouse hippocampus. Using such protocols, information regarding gene expression and regulatory molecular mechanisms from the same animal can be integrated and correlated with neurobiological and behavioral outcomes.

本文言語English
論文番号e33
ジャーナルCurrent Protocols
1
2
DOI
出版ステータスPublished - 2021 2月

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)
  • 神経科学(全般)
  • 免疫学および微生物学(全般)
  • 健康情報学
  • 医療検査技術
  • 医学(全般)

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