Common marmoset embryonic stem cell can differentiate into cardiomyocytes

Hao Chen, Fumiyuki Hattori, Mitsushige Murata, Weizhen Li, Shinsuke Yuasa, Takeshi Onizuka, Kenichiro Shimoji, Yohei Ohno, Erika Sasaki, Kensuke Kimura, Daihiko Hakuno, Motoaki Sano, Shinji Makino, Satoshi Ogawa, Keiichi Fukuda

研究成果: Article

11 引用 (Scopus)

抄録

Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.

元の言語English
ページ(範囲)801-806
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
369
発行部数3
DOI
出版物ステータスPublished - 2008 5 9

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Callithrix
Embryonic Stem Cells
Stem cells
Cardiac Myocytes
Bromodeoxyuridine
Germ Layers
Isoproterenol
Sinoatrial Node
Regenerative Medicine
Macaca fascicularis
Assays
Macaca mulatta
Primates
Action Potentials
Breeding
Haplorhini
Staining and Labeling
Cell Line
Research

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Common marmoset embryonic stem cell can differentiate into cardiomyocytes. / Chen, Hao; Hattori, Fumiyuki; Murata, Mitsushige; Li, Weizhen; Yuasa, Shinsuke; Onizuka, Takeshi; Shimoji, Kenichiro; Ohno, Yohei; Sasaki, Erika; Kimura, Kensuke; Hakuno, Daihiko; Sano, Motoaki; Makino, Shinji; Ogawa, Satoshi; Fukuda, Keiichi.

:: Biochemical and Biophysical Research Communications, 巻 369, 番号 3, 09.05.2008, p. 801-806.

研究成果: Article

Chen, H, Hattori, F, Murata, M, Li, W, Yuasa, S, Onizuka, T, Shimoji, K, Ohno, Y, Sasaki, E, Kimura, K, Hakuno, D, Sano, M, Makino, S, Ogawa, S & Fukuda, K 2008, 'Common marmoset embryonic stem cell can differentiate into cardiomyocytes', Biochemical and Biophysical Research Communications, 巻. 369, 番号 3, pp. 801-806. https://doi.org/10.1016/j.bbrc.2008.02.141
Chen, Hao ; Hattori, Fumiyuki ; Murata, Mitsushige ; Li, Weizhen ; Yuasa, Shinsuke ; Onizuka, Takeshi ; Shimoji, Kenichiro ; Ohno, Yohei ; Sasaki, Erika ; Kimura, Kensuke ; Hakuno, Daihiko ; Sano, Motoaki ; Makino, Shinji ; Ogawa, Satoshi ; Fukuda, Keiichi. / Common marmoset embryonic stem cell can differentiate into cardiomyocytes. :: Biochemical and Biophysical Research Communications. 2008 ; 巻 369, 番号 3. pp. 801-806.
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