抄録
Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 801-806 |
| ページ数 | 6 |
| ジャーナル | Biochemical and Biophysical Research Communications |
| 巻 | 369 |
| 発行部数 | 3 |
| DOI | |
| 出版物ステータス | Published - 2008 5 9 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
これを引用
Common marmoset embryonic stem cell can differentiate into cardiomyocytes. / Chen, Hao; Hattori, Fumiyuki; Murata, Mitsushige; Li, Weizhen; Yuasa, Shinsuke; Onizuka, Takeshi; Shimoji, Kenichiro; Ohno, Yohei; Sasaki, Erika; Kimura, Kensuke; Hakuno, Daihiko; Sano, Motoaki; Makino, Shinji; Ogawa, Satoshi; Fukuda, Keiichi.
：: Biochemical and Biophysical Research Communications, 巻 369, 番号 3, 09.05.2008, p. 801-806.研究成果: Article
}
TY - JOUR
T1 - Common marmoset embryonic stem cell can differentiate into cardiomyocytes
AU - Chen, Hao
AU - Hattori, Fumiyuki
AU - Murata, Mitsushige
AU - Li, Weizhen
AU - Yuasa, Shinsuke
AU - Onizuka, Takeshi
AU - Shimoji, Kenichiro
AU - Ohno, Yohei
AU - Sasaki, Erika
AU - Kimura, Kensuke
AU - Hakuno, Daihiko
AU - Sano, Motoaki
AU - Makino, Shinji
AU - Ogawa, Satoshi
AU - Fukuda, Keiichi
PY - 2008/5/9
Y1 - 2008/5/9
N2 - Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.
AB - Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.
KW - Cardiomyocytes
KW - Characterization
KW - Common marmoset
KW - Differentiation
KW - Embryonic stem cell
KW - Heart regeneration
KW - Monkey
KW - Preclinical model
KW - Primate
UR - http://www.scopus.com/inward/record.url?scp=41149142242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41149142242&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.02.141
DO - 10.1016/j.bbrc.2008.02.141
M3 - Article
C2 - 18331825
AN - SCOPUS:41149142242
VL - 369
SP - 801
EP - 806
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -