Common T cell receptor clonotype in lacrimal glands and labial salivary glands from patients with Sjögren's syndrome

Isao Matsumoto, Kazuo Tsubota, Yoshiyuki Satake, Yasuhiko Kita, Ryutaro Matsumura, Hideyuki Murata, Takashi Namekawa, Kusuki Nishioka, Itsuo Iwamoto, Yasushi Saitoh, Takayuki Sumida

研究成果: Article査読

86 被引用数 (Scopus)

抄録

Sjögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration into lacrimal and salivary glands leading to symptomatic dry eyes and mouth. Immunohistological studies have clarified that the majority of infiltrating lymphocytes around the lacrimal glands and labial salivary glands are CD4 positive αβ T cells. To analyze the pathogenesis of T cells infiltrating into lacrimal and labial salivary glands, we examined T cell clonotype of these cells in both glands from four SS patients using PCR-single-strand conformation polymorphism (SSCP) and a sequencing method. SSCP analysis showed that some infiltrating T cells in both glands expand clonally, suggesting that the cells proliferate by antigen-driven stimulation. Intriguingly, six to sixteen identical T cell receptor (TCR) Vβ genes were commonly found in lacrimal glands and labial salivary glands from individual patients. This indicates that some T cells infiltrating into both glands recognize the shared epitopes on autoantigens. Moreover, highly conserved amino acid sequence motifs were found in the TCR CDR3 region bearing the same TCR Vβ family gene from four SS patients, supporting the notion that the shared epitopes on antigens are limited. In conclusion, these findings suggest that some autoreactive T cells infiltrating into the lips and eyes recognize restricted epitopes of a common autoantigen in patients with SS.

本文言語English
ページ(範囲)1969-1977
ページ数9
ジャーナルJournal of Clinical Investigation
97
8
DOI
出版ステータスPublished - 1996 4月 15
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

フィンガープリント

「Common T cell receptor clonotype in lacrimal glands and labial salivary glands from patients with Sjögren's syndrome」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル